Single Nucleotide Polymorphisms in Skeletal Muscles of Myogenin and Myostatin Genes of the Nile Tilapia (Oreochromis niloticus)

The present study was conducted on the Nile tilapia (Oreochromis niloticus) and screened for single nucleotide polymorphisms (SNPs) in muscle hormone genes myogenin (MyoG) myostatin (MSTN). Samples were collected from JangHang fish farm categorized by body weight sex. average total 1.02 kg 82 males 0.63 70 females. A range of ±10% considered as normal weight. Target regions each gene categorized, such promoter region, 5′-untranslated region (5′UTR) 3′UTR. Genomic deoxyribonucleic acid (gDNA) extracted, amplified, sequenced, aligned, presence variations. Three SNPs, 1,875T>A, 1,892C>T, 1,946A>G, one insertion (TGA​CAT​ATT​TCC​CTC​ACA) at 2,138–2,145 detected 3′UTR MyoG gene. However, within MSTN gene, 450 (T) two SNPs 536 (G>A A>A) found 5′UTR. In addition, four insertions 1,278–1,279 (GT, T, TGT, G) short tandem repeat (STR) 1,129 (TG) Exon 1, 4,438 (3′UTR; A>C C>C), 4,514 G>A identified. Principal component analysis (PCA) statistical analyses performed to evaluate relationships between weight, forming 6 41 genotype blocks genes, respectively. We significant correlations traits effective GBG-1, GBG-3, GBG-4 GBN-1, GBN-2, GBN-3, GBN-4, GBN-13, GBN-22, GBN-24 MSTN. These outcomes would be breeding may help obtain data use genetic marker kits. some instances, kits have a distinct advantage improvement genetics, parentage control, identification species, enhancing stock its applications aquaculture.